Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.019121 |
Chromosome: | chromosome 9 |
Location: | 7198748 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g412250 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTTATCGGTTGTTACAAT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 751 |
LEAP-Seq percent confirming: | 97.0588 |
LEAP-Seq n confirming: | 33 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTGTGTCCGTAAGTGCCA |
Suggested primer 2: | AGGCAGTGAGTGGCAGAACT |