| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.019233 |
| Chromosome: | chromosome 6 |
| Location: | 606606 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253300 | UBCH1 | (1 of 1) K05610 - ubiquitin carboxyl-terminal hydrolase L5 [EC:3.4.19.12] (UCHL5, UCH37) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTGTGCCAGCATGAGCA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1259 |
| LEAP-Seq percent confirming: | 96.3542 |
| LEAP-Seq n confirming: | 370 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATACCATGGCAACTATG |
| Suggested primer 2: | CCGCTGTAAAACAGGATGGT |