Insertion junction: LMJ.SG0182.019295_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.019295
Chromosome:chromosome 7
Location:1604376
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g7506 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGGAGGTGGTGCATGGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1311
LEAP-Seq percent confirming:98.422
LEAP-Seq n confirming:1684
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGCGGTGTAGGTTGTAG
Suggested primer 2:ACTGCCCTTGATCTGGTCAC