Insertion junction: LMJ.SG0182.019308_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.019308
Chromosome:chromosome 3
Location:6709455
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g197600 (1 of 1) PTHR12972//PTHR12972:SF0 - DOWNSTREAM NEIGHBOR OF SON // PROTEIN DOWNSTREAM NEIGHBOR OF SON 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACTTGCCTTCTAACCAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGACAAGACAGGGTTGG
Suggested primer 2:AACCAGTGAGGTGGAGGTTG