| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.019326 |
| Chromosome: | chromosome 2 |
| Location: | 3592733 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| g1910 | Pyruvate-ferredoxin oxidoreductase | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCCGGCTTCCGGCGCA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1292 |
| LEAP-Seq percent confirming: | 99.8981 |
| LEAP-Seq n confirming: | 980 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTGCTGCAATTCTTGTC |
| Suggested primer 2: | ATGGTATGTAGGCAGGGCAG |