Insertion junction: LMJ.SG0182.019398_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019398
Chromosome:chromosome 8
Location:72095
Confidence (%):75
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCTACATCTGCATTCTAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:638
LEAP-Seq percent confirming:99.6
LEAP-Seq n confirming:249
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGATCGTGGTGTTACTCT
Suggested primer 2:AGCGGACCATTACACACACA