Insertion junction: LMJ.SG0182.019401_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.019401
Chromosome:chromosome 6
Location:2110597
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g265300 CSS2 Chlamydomonas specific family S protein; (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGCCCATCGCTGCACGAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:714
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:169
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAATGGTGTGTTGGCTGT
Suggested primer 2:TGCAGGCAAGACGATAGTTG