Insertion junction: LMJ.SG0182.019421_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019421
Chromosome:chromosome 6
Location:5625335
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g285900 FAP396 (1 of 2) IPR000104//IPR003590 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 396 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCATACACCCAATGGGAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGGAAGAGGGGAAGAAG
Suggested primer 2:CCAGCCCAAACTAAACCAAA