Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.019500 |
Chromosome: | chromosome 11 |
Location: | 1014815 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
g11596 | LCI13 | Low-CO2-inducible protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAATTAGTAGTCGAGCA |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1418 |
LEAP-Seq percent confirming: | 99.4355 |
LEAP-Seq n confirming: | 1233 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCGCGTCTTCAAACTCTT |
Suggested primer 2: | ACGCACCTCGTTACTATGGG |