Insertion junction: LMJ.SG0182.019500_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019500
Chromosome:chromosome 11
Location:1014815
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g11596 LCI13 Low-CO2-inducible protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAAATTAGTAGTCGAGCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1418
LEAP-Seq percent confirming:99.4355
LEAP-Seq n confirming:1233
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGCGTCTTCAAACTCTT
Suggested primer 2:ACGCACCTCGTTACTATGGG