Insertion junction: LMJ.SG0182.019553_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.019553
Chromosome:chromosome 11
Location:2561652
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre11.g476200 NZF1 (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH); CCCH-type zinc finger protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCACCACACGCGATACCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:667
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCAACGTGTCCTCCACC
Suggested primer 2:ACTCACCAACCCGCAATTAG