Insertion junction: LMJ.SG0182.019627_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.019627
Chromosome:chromosome 6
Location:2895691
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g272250 PHC27 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCACAGTCTTGGTCAGCCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:772
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTCCAGCTGCATCGACTT
Suggested primer 2:TGACAAACACATCCCCTCAA