Insertion junction: LMJ.SG0182.019663_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.019663
Chromosome:chromosome 3
Location:8009619
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g201550 MMP20 Matrix metalloproteinase; (1 of 1) IPR000095//IPR008752 - CRIB domain // Peptidase M11, gametolysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGAAAGCACATGTTACC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1439
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:775
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGAACAAGGACACCGTC
Suggested primer 2:AAATGGCACTACAGCATCCC