Insertion junction: LMJ.SG0182.019765_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019765
Chromosome:chromosome 6
Location:6821998
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g295100 EEP4 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGCTGCGAGACAGGAAGAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1344
LEAP-Seq percent confirming:85.6522
LEAP-Seq n confirming:591
LEAP-Seq n nonconfirming:99
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAATGCTGTTGTCCTCCA
Suggested primer 2:CAACCAGCGCTACAGCTACA