Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.019910 |
Chromosome: | chromosome_8 |
Location: | 2519694 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g372100 | HSP70A | Heat shock protein 70A | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGACAGGCGGCCCTTGTC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 582 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 91 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCCATCGACTCCTTGTCC |
Suggested primer 2: | GGCAAGGAGCTGAACAAGTC |