Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.SG0182.019940 |
Chromosome: | chromosome_14 |
Location: | 3440671 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
g15189 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAGCGTCAAGCAGATCTCTA |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 581 |
LEAP-Seq percent confirming: | 92.7711 |
LEAP-Seq n confirming: | 77 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGCTCCTCCAAAGTACCA |
Suggested primer 2: | ACGTGCACACACACACACAC |