Insertion junction: LMJ.SG0182.019940_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.019940
Chromosome:chromosome_14
Location:3440671
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g15189 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCAGCGTCAAGCAGATCTCTA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:92.7711
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTCCTCCAAAGTACCA
Suggested primer 2:ACGTGCACACACACACACAC