Insertion junction: LMJ.SG0182.019978_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019978
Chromosome:chromosome_5
Location:2124272
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g5360 antisense MULTIPLE_SPLICE_VARIANTS

Insertion site details

Flanking sequence (orientation from cassette outwards):CCACCCAGGCTGTTTTCCCCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:912
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGTTTGAGCTGGCATTTG
Suggested primer 2:GTCGAAAGTCGTTTTCGCTC