Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.019978 |
Chromosome: | chromosome_5 |
Location: | 2124272 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
g5360 | antisense | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCACCCAGGCTGTTTTCCCCC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 912 |
LEAP-Seq percent confirming: | 91.6667 |
LEAP-Seq n confirming: | 44 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAGTTTGAGCTGGCATTTG |
Suggested primer 2: | GTCGAAAGTCGTTTTCGCTC |