Insertion junction: LMJ.SG0182.019991_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.019991
Chromosome:chromosome_7
Location:5177002
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCGCAAGCAAAATACGAAGAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1174
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:325
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGGAGTGTCACGTTATTT
Suggested primer 2:GAAGCTAGGTGGCATGAAGC