Insertion junction: LMJ.SG0182.020021_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020021
Chromosome:chromosome_7
Location:1182040
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g320900 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ACAGCCCACCCTCGCCAGTA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:997
LEAP-Seq percent confirming:98.4946
LEAP-Seq n confirming:1374
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGGCTTCAAACATCACT
Suggested primer 2:GGCCGGTACTTACATGTGCT