| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.020050 |
| Chromosome: | chromosome_5 |
| Location: | 2610349 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g236250 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCACATGCTCACGCCGTCC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1257 |
| LEAP-Seq percent confirming: | 84.9673 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGTGGAGCAGCAGTAG |
| Suggested primer 2: | AGATTGCACATACCTTGGGC |