Insertion junction: LMJ.SG0182.020050_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020050
Chromosome:chromosome 5
Location:2610349
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre05.g236250 Conserved WD40-repeat Protein; (1 of 2) PTHR22847//PTHR22847:SF459 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACATGCTCACGCCGTCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1257
LEAP-Seq percent confirming:84.9673
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGTGGAGCAGCAGTAG
Suggested primer 2:AGATTGCACATACCTTGGGC