Insertion junction: LMJ.SG0182.020050_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.020050
Chromosome:chromosome_5
Location:2610349
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre05.g236250 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GAGGCCGCTGTTGCTGCTGCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:134
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGTGGAGCAGCAGTAG
Suggested primer 2:AGATTGCACATACCTTGGGC