| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.020079 |
| Chromosome: | chromosome_12 |
| Location: | 3446803 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| g12657 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCAGCCCGAGCACCCCGC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1394 |
| LEAP-Seq percent confirming: | 99.169 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACACCTGCTGCGATGAC |
| Suggested primer 2: | GCTGCAAGAACGAATTCACA |