Insertion junction: LMJ.SG0182.020085_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.020085
Chromosome:chromosome 1
Location:5494449
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre01.g038700 CLV5 Voltage-gated chloride channel; (1 of 2) K05016 - chloride channel 7 (CLCN7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGCAAGTTGACACTCCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:913
LEAP-Seq percent confirming:98.5714
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAGATGCGTGTATCCAA
Suggested primer 2:TCCTGGCTTCTCTACGCAAT