Insertion junction: LMJ.SG0182.020086_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.020086
Chromosome:chromosome_12
Location:4392611
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCCAAAGCAGCGCGCGTTC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:828
LEAP-Seq percent confirming:98.1013
LEAP-Seq n confirming:155
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTTGGGTTCAGGTGGAG
Suggested primer 2:ATACTGTATCCGCACCCTCG