Insertion junction: LMJ.SG0182.020097_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020097
Chromosome:chromosome_2
Location:1418686
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g083450 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCGCCGGCGGGGAGCTGGTC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:833
LEAP-Seq percent confirming:20.1754
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:182
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGGTGAGATACGTGAGCG
Suggested primer 2:TGCATGAACTTACTGGCTCG