Insertion junction: LMJ.SG0182.020130_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.020130
Chromosome:chromosome_10
Location:585421
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):GGACGAGTGGCATTCGAGTA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:859
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACAAGCCTTGGAGTTCG
Suggested primer 2:CATTCGGTCCCTTCAGATGT