Insertion junction: LMJ.SG0182.020135_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.020135
Chromosome:chromosome_4
Location:1608280
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g4713 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CCGCCCTACCTGGTCAGCAAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAACGAGGTTGGAAATGGTT
Suggested primer 2:CGCTACTGAGACGGAGGAAC