| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.020149 |
| Chromosome: | chromosome_3 |
| Location: | 79691 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| g2855 | FBB7 | Flagellar/basal body protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGGAGGACCCCACCTGCC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGTTAGCACCATGTACC |
| Suggested primer 2: | CGGAGTCGTAAGCTGGAGTC |