Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.SG0182.020189 |
Chromosome: | chromosome_12 |
Location: | 8282645 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
g13546 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGCTTCGTAGCAAGCAAAC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 246 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 15 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTACTACGGCTCGTTCAGC |
Suggested primer 2: | AGGACCGCGTATGTAGGTTG |