Insertion junction: LMJ.SG0182.020189_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.020189
Chromosome:chromosome_12
Location:8282645
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g13546 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGCTTCGTAGCAAGCAAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACTACGGCTCGTTCAGC
Suggested primer 2:AGGACCGCGTATGTAGGTTG