Insertion junction: LMJ.SG0182.020222_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.020222
Chromosome:chromosome_12
Location:9295147
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g541150 sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTATGTAGCTTTTGGCTTGAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:645
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCTGCTAATATGGCCGTG
Suggested primer 2:GCGTGTGTTTGTTGATACGC