Insertion junction: LMJ.SG0182.020223_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020223
Chromosome:chromosome_7
Location:3025709
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g333450 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TCACCAGAGGGCAGCAACACC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1149
LEAP-Seq percent confirming:98.7654
LEAP-Seq n confirming:160
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCAAACACAAGTGAGGA
Suggested primer 2:CAAGACGCTGAGAGGGGTAG