Insertion junction: LMJ.SG0182.020227_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020227
Chromosome:chromosome_2
Location:6412230
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g2391 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GAGATTCGACAACAGTTCCCG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:825
LEAP-Seq percent confirming:99.0
LEAP-Seq n confirming:198
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAACACCAGCATTGATGACC
Suggested primer 2:CTGCATTGGTGTCCTCCTCT