| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.020231 |
| Chromosome: | chromosome_1 |
| Location: | 2122091 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| g286 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAAATGTTCACTACGAAATA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 99.8258 |
| LEAP-Seq n confirming: | 573 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTTCAGTTTCCTGTCC |
| Suggested primer 2: | CATGTCGACACGAGTTCACC |