Insertion junction: LMJ.SG0182.020231_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020231
Chromosome:chromosome_1
Location:2122091
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g286 antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ACAAATGTTCACTACGAAATA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1003
LEAP-Seq percent confirming:99.8258
LEAP-Seq n confirming:573
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTTCAGTTTCCTGTCC
Suggested primer 2:CATGTCGACACGAGTTCACC