Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.020249 |
Chromosome: | chromosome_7 |
Location: | 1969013 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
g7568 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAACACCAGCCCAGCCCCCCA |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 509 |
LEAP-Seq percent confirming: | 86.9565 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGACAGTGTATGCGGTGCAG |
Suggested primer 2: | TGTGTGTGTAAGGGAGCGAG |