Insertion junction: LMJ.SG0182.020249_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020249
Chromosome:chromosome_7
Location:1969013
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
g7568 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AAACACCAGCCCAGCCCCCCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:509
LEAP-Seq percent confirming:86.9565
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGACAGTGTATGCGGTGCAG
Suggested primer 2:TGTGTGTGTAAGGGAGCGAG