| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.020321 |
| Chromosome: | chromosome 5 |
| Location: | 902709 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247350 | (1 of 30) IPR000253 - Forkhead-associated (FHA) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCCAGCCAATGCTGCCGT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.2248 |
| LEAP-Seq n confirming: | 1152 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATTCCCAACGTGTCAAG |
| Suggested primer 2: | CTCCTCCTGGGAGGGTTTAC |