Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.020343 |
Chromosome: | chromosome 17 |
Location: | 4824312 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g733950 | CGL152 | (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Conserved in the Green Lineage | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGCGGAGGCTCCGGTGC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 518 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 15 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTTGAGCTGGATGTTGTT |
Suggested primer 2: | AAGGAGAGTCCACCTGCTGA |