Insertion junction: LMJ.SG0182.020343_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.020343
Chromosome:chromosome 17
Location:4824312
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre17.g733950 CGL152 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGCGGAGGCTCCGGTGC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTGAGCTGGATGTTGTT
Suggested primer 2:AAGGAGAGTCCACCTGCTGA