| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.051903_1 | Cre01.g009500 | CDPK3,CDPK5 | Calcium/calmodulin-dependent protein kinase; (1 of 2) PF00069//PF13499 - Protein kinase domain (Pkinase) // EF-hand domain pair (EF-hand_7) | 3'UTR | 73 |
| LMJ.RY0402.051903_2 | Cre10.g433900 | (1 of 2) K10592 - E3 ubiquitin-protein ligase HUWE1 (HUWE1, MULE, ARF-BP1) | CDS | 58 | |
| LMJ.RY0402.051903_3 | Cre12.g504400 | (1 of 1) 2.3.1.22 - 2-acylglycerol O-acyltransferase / Monoglyceride acyltransferase | 5'UTR | 95 | |
| LMJ.RY0402.051903_4 | Cre12.g504400 | (1 of 1) 2.3.1.22 - 2-acylglycerol O-acyltransferase / Monoglyceride acyltransferase | 5'UTR | 95 | |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| Cisplatin, screen 3 of 4 (~10.0% improved growth) |
| Flagella, screen 2 of 6 (~20.0% improved growth) |
| Flagella, screen 4 of 6 (~40.0% growth defect) |
| Flagella, screen 5 of 6 (~20.0% growth defect) |
| Flagella, screen 6 of 6 (~50.0% growth defect) |
| Heat Shock, screen 1 of 1 (~10.0% growth defect) |
| High temperature, screen 5 of 7 (~10.0% improved growth) |
| LAT007G11, screen 1 of 1 (~30.0% growth defect) |
| LAT030E11, screen 1 of 1 (~20.0% growth defect) |
| LAT031E04, screen 1 of 1 (~30.0% growth defect) |
| LAT033G04, screen 1 of 1 (~20.0% growth defect) |
| LAT034B04, screen 1 of 1 (~10.0% growth defect) |
| LAT036A03, screen 1 of 1 (~70.0% growth defect) |
| LAT042B06, screen 1 of 1 (~10.0% growth defect) |
| Photosynthesis or light sensitivity, screen 11 of 12 (~40.0% growth defect) |
| Predator, screen 1 of 11 (~70.0% growth defect) |
| Predator, screen 2 of 11 (~60.0% growth defect) |
| Predator, screen 3 of 11 (~70.0% growth defect) |
| Predator, screen 4 of 11 (No growth detected) |
| Predator, screen 5 of 11 (No growth detected) |
| Predator, screen 6 of 11 (~50.0% growth defect) |
| Predator, screen 7 of 11 (No growth detected) |
| Predator, screen 8 of 11 (~40.0% growth defect) |
| Predator, screen 9 of 11 (~40.0% growth defect) |
| Predator, screen 10 of 11 (~30.0% growth defect) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.