| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051903 |
| Chromosome: | chromosome 10 |
| Location: | 2214522 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433900 | (1 of 2) K10592 - E3 ubiquitin-protein ligase HUWE1 (HUWE1, MULE, ARF-BP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTCATGGCCAAAGCCATCGTGGAGAG |
| Internal bar code: | ACAATGATCGTCAACTTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 97.3819 |
| LEAP-Seq n confirming: | 3050 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGAGGAAATGCTGATGT |
| Suggested primer 2: | ACACCAGCCACCACTAGTCC |