Mutant strain: LMJ.RY0402.140451

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.140451_1 Cre04.g213250 (1 of 2) K01256 - aminopeptidase N (pepN) intron 95
LMJ.RY0402.140451_2 Cre04.g213250 (1 of 2) K01256 - aminopeptidase N (pepN) intron 95
LMJ.RY0402.140451_3 Cre06.g303200 CDS 95
LMJ.RY0402.140451_4 Cre06.g303200 CDS 95
LMJ.RY0402.140451_5 Cre12.g515050 HLM18,SET4 putative N-methyltransferase; (1 of 1) PTHR13793:SF5 - HISTONE-LYSINE N-METHYLTRANSFERASE ATX4-RELATED 3'UTR 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Paromomycin, screen 1 of 6 (~10.0% improved growth)
Paromomycin, screen 2 of 6 (No phenotype detected)
Paromomycin, screen 3 of 6 (~20.0% improved growth)
Paromomycin, screen 4 of 6 (~10.0% improved growth)
Paromomycin, screen 5 of 6 (~30.0% improved growth)
Paromomycin, screen 6 of 6 (~40.0% improved growth)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.