| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140451 |
| Chromosome: | chromosome 4 |
| Location: | 1597774 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGAGAGGATAAGTCGCCCTTGCCTGCA |
| Internal bar code: | ACGGACTGATGGTTGCGCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 99.146 |
| LEAP-Seq n confirming: | 10449 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCGTAAACTCCTCCCTC |
| Suggested primer 2: | TGCCACGTGACATGACCTAT |