Mutant strain: LMJ.RY0402.173761

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.173761_1 Cre05.g248650 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron 73
LMJ.RY0402.173761_2 Cre10.g430900 (1 of 1) IPR000104//IPR007259 - Antifreeze protein, type I // Gamma-tubulin complex component protein CDS 58

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Hydroxyurea, screen 1 of 2 (~30.0% growth defect)
LAT003D02, screen 1 of 1 (~50.0% growth defect)
LAT003D07, screen 1 of 1 (~20.0% improved growth)
LAT030B09, screen 1 of 1 (~20.0% growth defect)
LAT030E11, screen 1 of 1 (~30.0% growth defect)
LAT032D03, screen 1 of 1 (No phenotype detected)
LAT032H10, screen 1 of 1 (~30.0% growth defect)
LAT034B04, screen 1 of 1 (~20.0% growth defect)
LAT034H07, screen 1 of 1 (~40.0% growth defect)
LAT035F03, screen 1 of 1 (~30.0% growth defect)
LAT035G03, screen 1 of 1 (~10.0% improved growth)
LAT035H07, screen 1 of 1 (~40.0% growth defect)
LAT041D10, screen 1 of 1 (~30.0% growth defect)
LAT042C03, screen 1 of 1 (~50.0% growth defect)
Limited growth in liquid media, screen 3 of 4 (~20.0% growth defect)
Limited growth in liquid media, screen 4 of 4 (No phenotype detected)
Low Termperature, screen 1 of 1 (~40.0% growth defect)
Photosynthesis or light sensitivity, screen 2 of 12 (~20.0% growth defect)
Photosynthesis or light sensitivity, screen 4 of 12 (~10.0% improved growth)
Zeocin, screen 5 of 5 (No phenotype detected)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.