Mutant strain: LMJ.RY0402.233989

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.233989_1 Cre03.g162900 (1 of 3) PTHR11017//PTHR11017:SF145//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN CDS 95
LMJ.RY0402.233989_2 Cre03.g162900 (1 of 3) PTHR11017//PTHR11017:SF145//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN CDS 95
LMJ.RY0402.233989_3 Cre04.g216850 TUA2 (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 2 intron 95
LMJ.RY0402.233989_4 Cre04.g216850 TUA2 (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 2 intron 95
LMJ.RY0402.233989_5 Cre06.g270550 CDS 73
LMJ.RY0402.233989_6 Cre06.g311450 intron 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

LAT034B04, screen 1 of 1 (No phenotype detected)
LAT034H07, screen 1 of 1 (No phenotype detected)
LAT035F03, screen 1 of 1 (~20.0% improved growth)
LAT041D11, screen 1 of 1 (~10.0% growth defect)
Paromomycin, screen 1 of 6 (No phenotype detected)
Paromomycin, screen 3 of 6 (~10.0% improved growth)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.