Mutant strain: LMJ.RY0402.252721

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.252721_1 Cre01.g008650 CDK1 Cyclin-dependent kinase; (1 of 12) 2.7.11.22//2.7.11.23 - Cyclin-dependent kinase / Cdk-activating protein kinase // [RNA-polymerase]-subunit kinase / CTD kinase CDS 58
LMJ.RY0402.252721_2 Cre06.g278174 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase intron 95
LMJ.RY0402.252721_3 Cre06.g278174 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase intron 95

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Cisplatin, screen 1 of 4 (~40.0% growth defect)
Cisplatin, screen 2 of 4 (~30.0% growth defect)
Cisplatin, screen 3 of 4 (~60.0% growth defect)
Cisplatin, screen 4 of 4 (~20.0% growth defect)
Methyl methanesulfonate, screen 3 of 4 (No phenotype detected)
Methyl methanesulfonate, screen 4 of 4 (No phenotype detected)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.