Mutant strain: LMJ.RY0402.254566

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.254566_1 Cre01.g052750 (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel intron 73
LMJ.RY0402.254566_2 Cre02.g102500 RAD51C DNA recombination protein; (1 of 1) K10870 - RAD51-like protein 2 (RAD51L2, RAD51C) intron 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Bleomycin sulfate, screen 1 of 2 (~30.0% growth defect)
Bleomycin sulfate, screen 2 of 2 (~20.0% growth defect)
Cisplatin, screen 1 of 4 (~70.0% growth defect)
Cisplatin, screen 2 of 4 (~30.0% growth defect)
Cisplatin, screen 3 of 4 (~60.0% growth defect)
Cisplatin, screen 4 of 4 (~60.0% growth defect)
Methyl methanesulfonate, screen 1 of 4 (~70.0% growth defect)
Methyl methanesulfonate, screen 3 of 4 (~50.0% growth defect)
Methyl methanesulfonate, screen 4 of 4 (~60.0% growth defect)
Paromomycin, screen 3 of 6 (No phenotype detected)
Zeocin, screen 2 of 5 (~80.0% growth defect)
Zeocin, screen 3 of 5 (~40.0% growth defect)
Zeocin, screen 5 of 5 (~20.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.