Gene: Cre03.g194850
Overview
| Common name: | MDH1,MDN1 |
| Defline: | NAD-dependent malate dehydrogenase 1, chloroplastic; (1 of 1) PTHR11540:SF14 - MALATE DEHYDROGENASE, CHLOROPLASTIC |
| Description: | Malate dehydrogenase (MDH) (= malic dehydrogenase) [EC:1.1.1.37]; NAD-dependent; sodium acetate-induced in Chlamydomonas reinhardtii; nuclear gene; gene product is localized in the chloroplast; Genbank entry U42979, as MDH2; present in thylakoid-enriched |
| Synonyms: | g4035.t1,MDH1,Cre03.g194850.t1.1,MDN1 |
| Chromosome: | chromosome 3 |
Protein Localization
A plasmid for expressing Cre03.g194850 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre03.g194850-Venus-3xFLAG
Imaged strain: CSI_LW09B6
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/pyrenoid/pyrenoid periphery enriched
chloroplast/homogeneous
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.060820_1 | CLIP2.060820 | 3'UTR | 80 | 6.1 |
| CLIP2.060820_2 | CLIP2.060820 | 3'UTR | 80 | 6.1 |
| CLIP2.065156_1 | CLIP2.065156 | 3'UTR | 80 | 6.1 |
| CLIP2.065156_2 | CLIP2.065156 | 3'UTR | 80 | 6.1 |
| CLIP2.066477_1 | CLIP2.066477 | outside_mRNA | 80 | 6.1 |
| LMJ.RY0402.220840_1 | LMJ.RY0402.220840 | 3'UTR | 73 | 5.5 |
Gene Annotations
| PFAM: | PF00056, PF02866 |
| PANTHER: | PTHR11540 PTHR11540:SF14 |
| KEGG_ec: | EC:1.1.1.37 |
| KEGG_orthology: | K00026 |
| KOG: | KOG1494 |
| Gene ontology terms:: | GO:0003824, GO:0005975, GO:0006108, GO:0016491, GO:0016616, GO:0019752, GO:0030060, GO:0055114 |
| Best arabidopsis TAIR name: | AT3G47520 |
| Best arabidopsis TAIR symbol: | MDH |
| Best arabidopsis TAIR defline: | malate dehydrogenase |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.