| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066477 |
| Chromosome: | chromosome 3 |
| Location: | 6612794 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194850 | MDH1,MDN1 | NAD-dependent malate dehydrogenase 1, chloroplastic; (1 of 1) PTHR11540:SF14 - MALATE DEHYDROGENASE, CHLOROPLASTIC | outside_mRNA |
| Cre03.g194900 | CYA4 | Lipocalin-like adenylate cyclase; (1 of 3) PTHR11017//PTHR11017:SF145//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGGGCCTTGCATGGGCACACAGAAGGGCACACGCGGGCGCCCACCT |
| Internal bar code: | CTCAACCAAAGACTTGAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2579 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCTTCCTGTGGAGTT |
| Suggested primer 2: | ATTGTGATGGACAGGCAGGG |