| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220840 |
| Chromosome: | chromosome 3 |
| Location: | 6466694 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194850 | MDH1,MDN1 | NAD-dependent malate dehydrogenase 1, chloroplastic; (1 of 1) PTHR11540:SF14 - MALATE DEHYDROGENASE, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGAGAATTGGATGATGGCCACACAAT |
| Internal bar code: | GGGGCTTCCACTCGGCGTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 92.414 |
| LEAP-Seq n confirming: | 3411 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACAGCGAGCTAATAAGG |
| Suggested primer 2: | TTGTAATCCGGAGCTGAGGT |