Gene: Cre07.g322950
Overview
| Common name: | NADK1,NDK1 |
| Defline: | ATP-NAD kinase; (1 of 1) PTHR20275//PTHR20275:SF6 - FAMILY NOT NAMED // NADH KINASE POS5, MITOCHONDRIAL |
| Description: | NAD kinase (ATP:NAD 2'-phosphotransferase, EC 2.7.1.23), an enzyme found in both prokaryotes and eukaryotes, generates the important pyridine nucleotide NADP from substrates ATP and NAD; similar to Arabidopsis NADK, the Chlamydomonas enzyme lacks the N-terminal domain found in the NADK2 isoform, including its calmodulin-binding site; probably chloroplast-located; null-allele mutant was isolated (PMID 29743196) |
| Synonyms: | NDK1,g7461.t1,NADK1,Cre07.g322950.t1.1 |
| Chromosome: | chromosome 7 |
Protein Localization
A plasmid for expressing Cre07.g322950 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre07.g322950-Venus-3xFLAG
Imaged strain: CSI_LW08C2
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/homogeneous/pyrenoid depleted
shapes in cytoplasm/a few large puncta|vesicles in cytoplasm
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.003332_1 | CLIP2.003332 | 3'UTR | 80 | 6.1 |
| CLIP2.003332_2 | CLIP2.003332 | 3'UTR | 80 | 6.1 |
| CLIP2.004134_1 | CLIP2.004134 | CDS | 80 | 6.1 |
| CLIP2.004134_2 | CLIP2.004134 | CDS | 80 | 6.1 |
| CLIP2.006085_1 | CLIP2.006085 | intron | 80 | 6.1 |
| CLIP2.006085_2 | CLIP2.006085 | intron | 80 | 6.1 |
| CLIP2.007521_1 | CLIP2.007521 | 3'UTR | 63 | 6.1 |
| LMJ.RY0402.073138_1 | LMJ.RY0402.073138 | 3'UTR | 58 | 5.5 |
| LMJ.RY0402.158974_1 | LMJ.RY0402.158974 | intron | 73 | 5.5 |
| LMJ.RY0402.198819_1 | LMJ.RY0402.198819 | 3'UTR | 73 | 5.5 |
| LMJ.SG0182.019683_1 | LMJ.SG0182.019683 | intron | 75 | 5.3 |
| LMJ.SG0182.019683_2 | LMJ.SG0182.019683 | intron | 75 | 5.3 |
Gene Annotations
| PFAM: | PF01513 |
| PANTHER: | PTHR20275 PTHR20275:SF6 |
| KEGG_ec: | EC:2.7.1.23 |
| KEGG_orthology: | K00858 |
| KOG: | KOG2178 |
| Gene ontology terms:: | GO:0003951, GO:0006741, GO:0008152 |
| Best arabidopsis TAIR name: | AT3G21070 |
| Best arabidopsis TAIR symbol: | ATNADK2,NADK2 |
| Best arabidopsis TAIR defline: | NAD kinase 1 |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.