| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073138 |
| Chromosome: | chromosome 7 |
| Location: | 1424921 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322950 | NADK1,NDK1 | ATP-NAD kinase; (1 of 1) PTHR20275//PTHR20275:SF6 - FAMILY NOT NAMED // NADH KINASE POS5, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTGCGGATGGTTCGCAGTTTTTGGTT |
| Internal bar code: | TGATCCTGGGTCGTATTTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.8592 |
| LEAP-Seq n confirming: | 4964 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGCACGGAGTTAGGAC |
| Suggested primer 2: | CCGTTTACCCGCTTCTTACA |