Gene: Cre07.g339750

Overview

Common name:HEM2,FeC,FEC1,HEMH,HEM15
Defline:Ferrochelatase; (1 of 1) 4.99.1.1 - Ferrochelatase / Protoheme ferro-lyase
Description:Ferrochelatase, chloroplast precursor (Protoheme ferro-lyase)(Heme synthetase); matches gi:13249285; PMID: 16306143 ; Interacts with bestrophin-1 a thylakoid membrane protein [PMID: 28938113]
Synonyms:HEM15,HEMH,HEM2,FeC,g7902.t2,Cre07.g339750.t1.1
Chromosome:chromosome 7

Protein Localization

A plasmid for expressing Cre07.g339750 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre07.g339750-Venus-3xFLAG

Imaged strain: CSI_LW07E12

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/pyrenoid depleted

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

Bait ID Bait Name Prey ID Prey Name Reference
Cre01.g050950 CHLP1,CHLP,LYL1 Cre07.g339750 HEM2,FeC,FEC1,HEMH,HEM15 Wang et al. 2023
Cre16.g662600 BST1,LCI11,LCI11C Cre07.g339750 HEM2,FeC,FEC1,HEMH,HEM15 Mackinder et al. 2017

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.001987_1 CLIP2.001987 intron 80 6.1
CLIP2.001987_2 CLIP2.001987 intron 80 6.1
CLIP2.002384_1 CLIP2.002384 intron 80 6.1
CLIP2.002384_2 CLIP2.002384 intron 80 6.1
CLIP2.004661_1 CLIP2.004661 outside_mRNA 80 6.1
CLIP2.005392_1 CLIP2.005392 5'UTR 80 6.1
CLIP2.006143_1 CLIP2.006143 5'UTR 80 6.1
CLIP2.006143_2 CLIP2.006143 5'UTR 80 6.1
CLIP2.007052_1 CLIP2.007052 3'UTR 80 6.1
CLIP2.007052_2 CLIP2.007052 3'UTR 80 6.1

Gene Annotations

PFAM:PF00762
PANTHER: PTHR11108 PTHR11108:SF5
KEGG_ec:EC:4.99.1.1
KEGG_orthology:K01772
KOG:KOG1321
Gene ontology terms::GO:0004325, GO:0006783
Best arabidopsis TAIR name:AT2G30390
Best arabidopsis TAIR symbol:ATFC-II,FC-II,FC2
Best arabidopsis TAIR defline:ferrochelatase 2
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.