| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001987 |
| Chromosome: | chromosome 7 |
| Location: | 3962336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339750 | HEM2,FeC,FEC1,HEMH,HEM15 | Ferrochelatase; (1 of 1) 4.99.1.1 - Ferrochelatase / Protoheme ferro-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAGGGGTTGTTGGGTGAGGTCGGGATTGGGGTCGGGATTGGGGGTC |
| Internal bar code: | ATGCGGTGGACTCGGGATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2783 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACGGAAAGCGAGTCGTT |
| Suggested primer 2: | TGGTTATGCTGGGGTTGCAA |